ISO/AWI 21686-2
Water quality — In vivo fluorescence measurement of the endocrine disrupting effects of natural water and wastewater using transgenic embryos — Part 2: Estrogen Activity (Oryzias latipes)
Reference number
ISO/AWI 21686-2
Edition 1
Working draft
ISO/AWI 21686-2
A working group has prepared a draft.


This document specifies a method for the determination of the estrogen activity of water and waste water by means of a reporter gene assay with genetically modified Oryzias latipes (O. latipes, medaka) eleutheroembryos. This method is applicable to: - fresh water; - waste water; - aqueous extracts and leachates; - eluates of sediments (fresh water); - pore water; - aqueous solutions of single substances or of chemical mixtures; - drinking water. NOTE 1: Extraction, pre-concentration or dilution of water samples can prove necessary The organism preparation methods (freeze-drying, extraction, mineralization) and quantification of the chemical substances do not fall within the scope of this document.

General information

  •  : Under development
    : New project registered in TC/SC work programme [20.00]
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  • ISO/TC 147/SC 5
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