Reference number
ISO 19040-3:2018
International Standard
ISO 19040-3:2018
Water quality — Determination of the estrogenic potential of water and waste water — Part 3: In vitro human cell-based reporter gene assay
Edition 1
2018-08
Read sample
ISO 19040-3:2018
66297
Published (Edition 1, 2018)
This publication was last reviewed and confirmed in 2024. Therefore this version remains current.

ISO 19040-3:2018

ISO 19040-3:2018
66297
Language
Format
CHF 173
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Abstract

This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay utilizing stably transfected human cells. This reporter gene assay is based on the activation of the human estrogen receptor alpha.

This method is applicable to:

— fresh water;

— waste water;

— aqueous extracts and leachates;

— eluates of sediments (fresh water);

— pore water;

— aqueous solutions of single substances or of chemical mixtures;

— drinking water;

— the limit of quantification (LOQ) of this method for the direct analysis of water samples is between 0,3 ng/l and 1 ng/l 17β-estradiol equivalents (EEQ) based on the results of the international interlaboratory trial (see Annex F). The upper working range was evaluated [based on the results of the international interlaboratory trial (see Table F.3)] up to a level of 75 ng EEQ/l. Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre concentration of water samples can prove necessary if their estrogenic potential is below the given LOQ.

General information

  •  : Published
     : 2018-08
    : International Standard confirmed [90.93]
  •  : 1
     : 40
  • ISO/TC 147/SC 5
    13.060.70 
  • RSS updates

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