ISO/TS 12869:2019
p
ISO/TS 12869:2019
70756
Indisponible en français

Résumé

 Preview

This document specifies a method for the detection and quantification of Legionella spp. and L. pneumophila using a quantitative polymerase chain reaction (qPCR). It specifies general methodological requirements, performance evaluation requirements, and quality control requirements.

Technical details specified in this document are given for information only. Any other technical solutions complying with the performance requirements are suitable.

NOTE 1 For performance requirements, see Clause 9.

This document is intended to be applied in the bacteriological investigation of all types of water (hot or cold water, cooling tower water, etc.), unless the nature and/or content of suspended matter and/or accompanying flora interfere with the determination. This interference can result in an adverse effect on both the detection limit and the quantification limit.

NOTE 2 For validation requirements, see 9.7.

The results are expressed as the number of genome units of Legionella spp. and/or L. pneumophila per litre of sample.

The method described in this document is applicable to all types of water. However, some additives, such as chemicals used for water treatment, can interfere with and/or affect the sensitivity of the method.

The qPCR methods do not give any information about the physiological state of the Legionella.


Informations générales 

  •  :  Publiée
     : 2019-04
  •  : 2
  •  : ISO/TC 147/SC 4 Méthodes microbiologiques
  •  :
    07.100.20 Microbiologie de l'eau

Acheter cette norme

fr
Format Langue
std 1 166 PDF + ePub
std 2 166 Papier
  • CHF166

Vous avez une question?

Consulter notre FAQ

Service à la clientèle
+41 22 749 08 88

Horaires d’ouverture:
De lundi à vendredi - 09:00-12:00, 14:00-17:00 (UTC+1)

Suivez l'actualité de l'ISO

Inscrivez-vous à notre Newsletter (en anglais) pour suivre nos actualités, points de vue et informations sur nos produits.